Identification of nonpoint sources of fecal pollution in coastal waters by using host-specific 16S ribosomal DNA genetic markers from fecal anaerobes
Abstract
We describe a new PCR-based method for distinguishing human and cow fecal contamination in coastal waters without culturing indicator organisms, and we show that the method can be used to track bacterial marker sequences in complex environments. We identified two human-specific genetic markers and f …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
RNA
bacteria
animals
humans
pubmed abstract
nih
national institutes of health
national library of medicine
research support
non-u.s. gov't
u.s. gov't
non-p.h.s.
adult
Child
DNA
Ribosomal
Sequence Analysis
polymerase chain reaction
Feces / microbiology*
Molecular Sequence Data
Phylogeny
Genetic Markers
Polymorphism
cattle
bifidobacterium / genetics
16s / genetics*
bacteroides / isolation & purification
species specificity
pmid:10742246
pmc92027
doi:10.1128/aem.66.4.1587-1594.2000
a e bernhard
k g field
anaerobic / classification
anaerobic / genetics
anaerobic / isolation & purification*
bacteroides / classification
bacteroides / genetics
bifidobacterium / classification
bifidobacterium / isolation & purification
dna restriction enzymes / metabolism
bacterial / genetics
ribosomal / genetics*
restriction fragment length
prevotella / classification
prevotella / genetics
prevotella / isolation & purification
water microbiology*
water pollution*
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Citation
ID:
111552
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