De novo transcriptome sequencing in Anopheles funestus using Illumina RNA-seq technology

De novo transcriptome sequencing in Anopheles funestus using Illumina RNA-seq technology

Crawford JE;Guelbeogo WM;Sanou A;Traoré A;Vernick KD;Sagnon N;Lazzaro BP;;
PloS one 2010 Vol. 5 pp. -
446
je2010plosde

Abstract

We developed a pipeline that makes de novo transcriptome sequencing possible in virtually any organism at a very reasonable cost ($6,300 in sequencing costs in our case). We anticipate that our approach could be used to develop genomic resources in a diversity of systems for which full genome sequen …

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