Evaluation of polymerase chain reaction for diagnosis of pneumococcal pneumonia
Abstract
To test the ability of the polymerase chain reaction (PCR) to detect Streptococcus pneumoniae in blood, we generated two sets of nested primers. The first defined 559-bp and 649-bp regions of the pneumolysin gene, and the second defined 445-bp and 553-bp regions of the autolysin gene. These nucleoti …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
humans
pubmed abstract
nih
national institutes of health
national library of medicine
DNA
sensitivity and specificity
Polymerase Chain Reaction / methods*
Base Sequence
Molecular Sequence Data
pneumonia
pneumococcal / diagnosis*
bacterial / analysis*
pmid:8253962
pmc265966
doi:10.1128/jcm.31.10.2661-2666.1993
k m rudolph
a j parkinson
l w mayer
bacteremia / diagnosis*
streptococcus pneumoniae / genetics*
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111926
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