Comparison of microplate- and bottle-based methods to age yeast for chronological life span assays.
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Comparison of microplate- and bottle-based methods to age yeast for chronological life span assays.

Kwong, Mandy Mun Yee;Lee, Jee Whu;Samian, Mohammed Razip;Watanabe, Nobumoto;Osada, Hiroyuki;Ong, Eugene Boon Beng;
Journal of microbiological methods 2019 pp. 105743
265
kwong2019comparisonjournal

Abstract

This study compared the chronological life span and survival Saccharomyces cerevisiae aged in a microplate or bottle, under different aeration and calorie restriction conditions. Our data shows that limited aeration in the microplate-aged culture contributed to slower outgrowth but extended yeast CLS compared to the bottle-aged culture.

Keywords

aeration saccharomyces cerevisiae calorie restriction chronological life span

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DOI:
S0167-7012(19)30721-3

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