Abstarct Shiitake mushroom [Lentinula edodes(Berk) Singer/Pegler] has the second production class among the most important edible mushrooms. For a long time this mushroom has draw attention due to its unique flavor and taste and also therapeutic properties. ِDue to the importance of this mushroom in the world and also the effect of substrate on the production of this medicinal mushroom, a research were conducted in randomized complete block design with 6 replications on 4 different substrate formulations including: 1) sawdust, wheat bran, millet; 2) sawdust, wheat bran, molasses, chalk, calcium super phosphate; 3)sawdust, wheat bran, tea waste; 4)sawdust, saccharose, citric acid, chalk and calcium carbonate on L.edodes production for determining the suitable substarte on early fruiting, yield, biological effeciency, average mushroom numbers and weights per block. Results showed that substrate formulation 1 caused earlier fruiting than others (58 days from incubation time), this formulation also had the highest yield (112/3 g/log) and biological effeciency (35/09%) between the others formula. mashroom number was the most in substrate formulation 1 (16/67 n/log) and the highest mushroom wieght was obtained from formulation 4 (14/33 g/per mushroom). there wasn’t any significant differences at (p≤0.05) between substrate formulation 1 and 2. Keywords: Shiitake, Substrate formulation, Yield, Biological effeciency, Mushroom number and weight