A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes
Abstract
We have developed a simple non-radioactive in situ hybridization procedure for tissue sections and cultured cells using digoxigenin-labelled cRNA probes. This protocol can be applied for the detection of various transcripts present at a wide range of expression levels in the central nervous system. …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
RNA
animals
pubmed abstract
nih
national institutes of health
national library of medicine
research support
non-u.s. gov't
Cells
Cultured
Rats
genetic*
Receptors
Messenger / genetics
transcription
messenger / metabolism
immunohistochemistry
ethanol
cerebellum / metabolism
octoxynol
pmid:7512949
doi:10.1007/bf00267823
n schaeren-wiemers
a gerfin-moser
bisbenzimidazole
digoxigenin
glial fibrillary acidic protein / metabolism
in situ hybridization*
inositol polyphosphate 5-phosphatases
myelin basic protein / genetics
myelin basic protein / metabolism
nerve tissue / metabolism*
phosphoric monoester hydrolases / genetics
phosphoric monoester hydrolases / metabolism
complementary*
glutamate / genetics
glutamate / metabolism
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Citation
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271390
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