identification of chemosensory genes based on the transcriptomic analysis of six different chemosensory organs in spodoptera exigua
Abstract
Insects have a complex chemosensory system that accurately perceives external chemicals and plays a pivotal role in many insect life activities. Thus, the study of the chemosensory mechanism has become an important research topic in entomology. Spodoptera exigua Hübner (Lepidoptera: Noctuidae) is a major agricultural polyphagous pest that causes significant agricultural economic losses worldwide. However, except for a few genes that have been discovered, its olfactory and gustatory mechanisms remain uncertain. In the present study, we acquired 144,479 unigenes of S. exigua by assembling 65.81 giga base reads from 6 chemosensory organs (female and male antennae, female and male proboscises, and female and male labial palps), and identified many differentially expressed genes in the gustatory and olfactory organs. Analysis of the transcriptome data obtained 159 putative chemosensory genes, including 24 odorant binding proteins (OBPs; 3 were new), 19 chemosensory proteins (4 were new), 64 odorant receptors (57 were new), 22 ionotropic receptors (16 were new), and 30 new gustatory receptors. Phylogenetic analyses of all genes and SexiGRs expression patterns using quantitative real-time polymerase chain reactions were investigated. Our results found that several of these genes had differential expression features in the olfactory organs compared to the gustatory organs that might play crucial roles in the chemosensory system of S. exigua, and could be utilized as targets for future functional studies to assist in the interpretation of the molecular mechanism of the system. They could also be used for developing novel behavioral disturbance agents to control the population of the moths in the future.
Keywords
Mass spectrometry
candida albicans
peripheral blood mononuclear cell
innate immunity
bivalve mollusc
metabolism
bemisia tabaci
reproduction
listeria monocytogenes
rna interference
soybean
transcriptome analysis
neuropeptide
abiotic stresses
stress resistance
pdi
lipopolysaccharides
protein content
leaf hair lengthagriculture (general)
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257726
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0x95644003c57E6F55A65596E3D9Eac6813e3566dA
Article ID:
257726
Unique Identifier:
10.3389/fphys.2018.00432
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Scimatic Chain (ID: 481)
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