occurence of antibiotic resistant enterococci on skin of teats and teat cups of milking machine

occurence of antibiotic resistant enterococci on skin of teats and teat cups of milking machine

;Anna Krebs Artimová;Viera Ducková;Miroslav Kročko
Sensors (Basel, Switzerland) 2013 Vol. 7 pp. 181-185
161
artimov2013potravinarstvooccurence

Abstract


The production of high-quality milk with low bacteriological counts begins at the farm and involves multiple factors related to cows, environment, and hygiene of equipment. The aim of this study was to investigate the occurrence of enterococci in conditions of farmhouse production and antibiotic resistance of 49 strains isolated from skin of teats and 50 strains isolated from teat cups of milking machine. The samples for enumeration and isolation of enterococci were cultured on selective diagnostic Slanetz-Bartley agar. The isolates were phenotypically identified at the species level with EN-COCCUS test. Susceptibilities of isolated enterococci to antibiotic (vancomycin 30 μg/disc, ampicillin 10 μg/disc, erythromycin 15 μg/disc, gentamicin 120 μg/disc, teicoplanin 30 μg/disc and tetracycline 30 μg/disc) were tested using the disc diffusion method.  The counts of enterococci from teats reached the average value 2.77 log10 cfu.ml-1 and from teat cups of milking machine average value 2.85 log10 cfu.ml-1. The dominant species isolated from teats (41%) and also from teat cups (36%) were representatives of E. group III - E. durans, E. hirae, E. faecalis asaccharolytic var. The isolates of enterococci obtained from teats showed resistance to tetracycline (4%), erythromycin (4%) and intermediary resistance to tetracycline (8%), erythromycin (25%) and to vancomycin (8%). The isolates of enterococci obtained from teat cups showed resistance to tetracycline (2%) and intermediary resistance to erythromycin (20%) and to vancomycin (6%). No resistance to the antibiotic teicoplanin, ampicillin and gentamicin was found. The most of the isolates of enterococci were sensitive to the tested antibiotics.

Citation

ID: 243304
Ref Key: artimov2013potravinarstvooccurence
Use this key to autocite in SciMatic or Thesis Manager

References

Blockchain Verification

Account:
NFT Contract Address:
0x95644003c57E6F55A65596E3D9Eac6813e3566dA
Article ID:
243304
Unique Identifier:
10.5219/310
Network:
Scimatic Chain (ID: 481)
Loading...
Blockchain Readiness Checklist
Authors
Abstract
Journal Name
Year
Title
5/5
Creates 1,000,000 NFT tokens for this article
Token Features:
  • ERC-1155 Standard NFT
  • 1 Million Supply per Article
  • Transferable via MetaMask
  • Permanent Blockchain Record
Blockchain QR Code
Scan with Saymatik Web3.0 Wallet

Saymatik Web3.0 Wallet