Researches done on a B.R. 64 milking cows live stock, regarding microbiological charge of cow milk and pollutant sources on technological flow,. Samples were taken in sterile test tubes, to study the microbian charge level from: milking empty canteen; gauze used for udder hygiene; first milking milk jets from the four quarters; total milking milk on lots; water used for mammary gland hygiene; after the first and after the last milking animal; cooling tank (milking milk on total /live stock). Laboratory analysis regarding microflora charge level of samples, evidence enough large variations regarding microbian charge level in samples collected.