Nocodazole (NCD) is an anti-neoplastic agent which exerts its effect in cells by interfering with the polymerization of microtubules. As NCD affects the cytoskclcton. it is often used inceli biology experiments as a control. In tlıis study, NCD was loaded into Solid Lipid Nanoparticle (SLN) Systems. A reverse plıase lıigh perfonnance liquid clıromatography (HPLC) method was validated and applied forthe detennination of NCD in SLN. Determination and validation studies were carried out on a 4.6 % 150 inin, 5pırı Cı8 Thenno column using an optimized mobile plıase (MP) of methanol:water:phosplıate buffer (45:42.5:12.5, v/v/v , pH 5.03) at a flow rate of 0.8 ınL/min. Diode array detection was perfonned at 256 mn and the column temperature was adjusted to 40°C. Naproxen was used as an intemal Standard (IS). The retention times for naproxen and NCD were 2.2 and 9.8 inin, respectively. The specifıed working range was derived from linearity studies and kept in the concentration range 0.5-100 ppm. Limit of dedection (LOD) and limit of quantitation (LOQ) values were detennined to be 0.065 ppm and 0.196 ppm, respectively. NCD recovery % results of the SLN fonnulations which are stored at 25°C, 4°C and 40°C were investigated and compared to the freslıly prepared samples