In the current study, antioxidant activities of the aerial parts and roots ofTragopogon longirostris Bisch. ex Sch. Bip. var. longirostris and T. pratensis subsp. orientalis L. were evaluated. Antioxidant activity of the Tragopogon aqueous methanol extracts were investigated by DPPH scavenging and superoxide anion scavenging assays. Total phenolic and flavonoid contents of the extracts were calculated using Folin-Ciocalteu and aluminum chloride colorimetric methods and determined ranging from 63.4510.25 to 68.92+0.42 mg/g caffeic acid and from 4±1 to 210+9 mg/g quercetin respectively. Furthermore the phenolic acids; chlorogenic acid, caffeic acid, ferulic acid, rosmarinic acid, p-coumaric acid and flavonoids; apigenin, luteolin, quercetin, hyperoside, rutin, hesperidin in T. longirostris var. longirostris and T. pratensis subsp. orientalis have been determined qualitatively and quantitatively using reverse phase high performance liquid chromatography. Chlorogenic acid w as determined in ali samples investigated. The highest content of chlorogenic acid w as detected in aerial part extract of T. longirostris var. longirostris as 578.22 ± 4.19 jug/mg