The aim of the paper was to study the evolution of the immunoglobulin types IgG, IgA and IgM concentrations during the first three days postpartum in Romanian Black and White cows. Samples of colostrum were collected at calving and at 4-hour interval for three days. Samples were analyzed for immunoglobulin concentration using ELISA test. The maximum concentration of immunoglobulin Ig G in colostral milk was found as expected at the first milking 43.8 mg/ml. At the third, fourth, fifth and sixth milking from the second day after calving, respectively at 36, 40, 44 and 48 hours after calving immunoglobulin type Ig G concentration decreased to 42.0, 41.3, 39.7 and 38.3 mg/ml. At the eighteenth milking from the third day after calving, respectively at 72 hours after calving immunoglobulin type Ig G concentration decreased to 15.2 mg/ml. The maximum concentration of immunoglobulin Ig A in colostral milk was found as expected at the first milking 4.05 mg/ml. The concentrations of immunoglobulin type Ig M decreased in the first day after calving from 4.8 mg/ml at first milking to 3.8 mg/ml at the sixth milking.