The effects of media temperature, agitation rate and molasses concentration on the yield of fermentation in xanthan gum production process were investigated. Xanthan gum was produced in batch fermentation by Xanthomonas campestris PTCC 1473 from molasses. At 32 C, 500 rpm and media with 30 g/l of total sugar, maximum production of xanthan gum (17.1 g/l) was achieved. For the purity of the xanthan FTIR spectrum was obtained. The identified spectrum was compared with the commercial product. In batch culture, several kinetic models for the biochemical reactions were extensively studied. The growth kinetic parameters were evaluated by unstructured model and derived from the related equations. Based on Malthus and Logistic rate equations, the maximum specific growth rate, max, and initial cell dry weight, X0, were defined. Luedeking-Piret and Modified Luedeking-Piret models were applied for the product formation and substrate consumption rates. In batch experiments, the kinetic parameters for the growth associated (m, a) and non-growth associated (n, b) parameters were determined.