The aim of this study was to determine the best planting practices for two bio fortified Orange Fleshed Sweet Potatoes (OFSP). The study investigated the effect of spacing (20, 30 and 40 cm) and maturity (12 and 16 weeks) period on the proximate composition, α-amylase, reducing sugar as well as total and soluble carbohydrates of two OFSP varieties (Umuspo1 and Ex-Onyunga). The moisture content of OFSP ranged from 68.30 to 82.67 % at 12 weeks and 63.73 to 74.67 % at 16 weeks, while the ash, fat, fibre, protein and carbohydrate at 12 weeks maturity period were from 0.47 to 1.16, 0.47 to 3.13, 0.41 to 4.41, 1.88 to 2.88 and 10.52 to 25.46 % respectively. On the other hand, ash, fat, fibre, protein and carbohydrate at 16 weeks maturity period ranged from 0.63 to 1.03, 0.47 to 3.13, 0.87 to 3.77, 1.58 to 2.44 and 13.32 to 30.07 % respectively. The results also indicated that Ex-Onyunga variety planted at 40 cm spacing harvested at the period of 12 weeks had the highest protein content (2.88%) which was significantly different (p