Rapid transcriptome characterization for a nonmodel organism using 454 pyrosequencing
Abstract
We present a de novo assembly of a eukaryote transcriptome using 454 pyrosequencing data. The Glanville fritillary butterfly (Melitaea cinxia; Lepidoptera: Nymphalidae) is a prominent species in population biology but had no previous genomic data. Sequencing runs using two normalized complementary D …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
animals
pubmed abstract
nih
national institutes of health
national library of medicine
research support
non-u.s. gov't
u.s. gov't
non-p.h.s.
sequence alignment
DNA
Sequence Analysis
Base Sequence
Polymorphism
DNA / methods*
complementary / genetics
oligonucleotide array sequence analysis
expressed sequence tags*
gene expression profiling*
single nucleotide
pmid:18266620
doi:10.1111/j.1365-294x.2008.03666.x
j cristobal vera
christopher w wheat
james h marden
alternative splicing
butterflies / genetics*
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113277
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