Gene discovery and annotation using LCM-454 transcriptome sequencing
Abstract
454 DNA sequencing technology achieves significant throughput relative to traditional approaches. More than 261,000 ESTs were generated by 454 Life Sciences from cDNA isolated using laser capture microdissection (LCM) from the developmentally important shoot apical meristem (SAM) of maize (Zea mays …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
pubmed abstract
nih
national institutes of health
national library of medicine
research support
non-u.s. gov't
u.s. gov't
non-p.h.s.
sequence alignment
DNA
Genes
Sequence Analysis
Base Sequence
Expressed Sequence Tags
DNA / methods*
plant*
pmid:17095711
pmc1716268
doi:10.1101/gr.5145806
scott j emrich
w brad barbazuk
patrick s schnable
complementary
lasers
meristem
zea mays / genetics*
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110589
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