Purpose: To evaluate the efficacy of surgical excision in combination with mitomycin C (mit-C) and postoperative topical mit-C 0.002% administration for the treatment of conjunctival intraepithelial neoplasia (CIN). Material and Method: Twelve eyes of twelve patients who were histopathologically diagnosed as CIN between March 2004 and July 2008 were evaluated retrospectively for their treatment modalities and results. All lesions were excised with wide margins in combination with mit-C 0.02% application for 2 minutes. Five of 12 eyes were treated with topical mit-C 0.002% eye drops four times a day for 14 days after histopathological confirmation. Large conjunctival defects in 5 eyes were reconstructed with amniotic membrane (3 eyes) and autologous conjunctiva (2 eyes). Results: Seven patients were women and 5 were men, with a mean age of 68.37 (range: 30-94) years. The right eye was involved in 6 patients and the left one - in 6 patients. The conjunctival lesion was located on the nasal conjunctiva in 5 eyes and on the temporal one - in 7 eyes. Histopathological diagnosis was CIN in all cases. After a mean follow-up period of 41.75 months (between 10 and 62 months), one recurrence (8.3%) was noted. This case with recurrent lesion has been previously treated with surgical excision in combination with mit-C 0.02% application and the large conjunctival defect has been reconstructed with amniotic membrane. Discussion: Surgical excision in combination with mit-C and use of additional postoperative topical mit-C 0.002% in selected cases for conjunctival intraepithelial treatment provides a safe and effective cure. (Turk J Ophthalmol 2011; 41: 138-42)