This study investigated the effects of dietary chitosan on the growth performance and nonspecific immunity of loaches (Misgurnus anguillicadatus). Four practical diets at three levels of chitosan (1, 5 and 10 g/kg) were fed to loach (3.13 ± 0.02 g) in triplicate groups (20 fish per replicate) for 10 weeks. Contrary to high-chitosan treatment, the administration of low or moderate levels of oral chitosan (1 or 5 g/kg) significantly increased the body weight gain, specific growth rate and condition factor. The oral administration of chitosan significantly increased the survival rate, phenoloxidase, superoxide dismutase, glutathione peroxidase, lysozyme, acid phosphatase and alkaline phosphatase activities, as well as the immunoglobulin M and complement 3 contents and the disease resistance against Aeromonas hydrophila. The optimum dose of dietary chitosan required for the maximum growth of loach was 5 g/kg. These results indicated that chitosan exerted immunostimulatory effects on loaches (M. anguillicadatus) and can thus be used as a dietary supplement.