Aim: To investigate the effect of age and gender on the degree of maxillary and mandibular anterior teeth display while speaking and smiling in Indian population. Materials and Method: A total of 120 subjects (63 females and 57 males) were included in this study. The subject was asked pronounced "ah"& "six" 3 times each sound, closing the mouth and resting between each sound. After these sounds, a smile was provoked. Each image was inserted into a separate slide of presentation software and measurements were taken. Results: There was a statistically significant difference in display of maxillary and mandibular anterior teeth owing to age and gender. Conclusion: Differences in tooth display in relation to aging and gender should be considered when providing esthetic treatment that involves replacement of anterior teeth.