The effect of susceptibility/resistance of seed potato varieties on population of golden cyst nematode – Globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 was investigated in the infested field of Brdo, KO Ljubovija No. 413, in Mačva District in Serbia during 2002 and 2003. The susceptible varieties Desiree, Innovator, Kennebec, Cleopatra, and Kondor were used in the experiment as varieties frequently grown in this district. The resistant ex-andigena varieties Agria, Frisia, Latona, Saturna, and Tresor were chosen on the basis of identified pathotypes of G. rostochiensis population originating from the aforementioned infested field. The results of this research indicated that nematode population of G. rostochiensis could be reduced and higher yield obtained by growing the resistant ex-andigena varieties in case of presence of pathotype Ro1. In the second year of investigation, final cyst density was 3.5 times lower than in the first year. It subsequently affected the crop yield which increased in the second year. The lowest crop yield was observed for susceptible varieties Kennebec (8.5 t/ha) and Cleopatra (16.9 t/ha), and the highest for resistant varieties Agria (25.7 t/ha) and Frisia (29.1 t/ha). These results may be useful to growers in the infested areas of Mačva District when choosing potato varieties, in order to eradicate the quarantine nematode G. rostochiensis.