Background: Candidiasis is a prevalent disease which is caused by different species of Candida. Herbal drugs (e.g. ginseng) were traditionally administrated for the treatment of different diseases. This study was carried out to compare the effect of alcoholic extract of ginseng with Itraconazole against Candida albicans (C. albicans) and Candida krusei (C. krusei). Material and Methods: This cross-sectional study was crried out on 22 and 8 species of C.albicans and 8 C.krusei, respectively which were isolated from vagina, urine and sputum of the patients. Using the CLSI M27 and disk diffusion methods the susceptibility test was done by Itraconazole (10 µg) and ginseng extract (1, 2, 4, 8, 16, 32, 64 and 128 mg.ml-1). The standard species of C. albicans (PTCC 5027) and C. Krusei (PTCC 5295) were used for the quality control purposes. Results: The lowest and highest minimum inhibitory concentration (MIC) for C. albicans and C. Kruzei was 0.0625 and 0.5 μg.ml-1, respectively for Itraconazole using the microdilution method. However, the lowest MIC and minimum fungal concentration (MFC) for alcoholic extract was 64 mg.ml-1 .The highest inhibition zone for C. albicans was 14 and 14-32 mm for alcoholic extract and Foritraconazole, respectively. Using the two methods no significant difference was seen between the alcoholic extract of ginseng (64 and 128 mg.ml-1) and the drug. (P<0.05) Conclusion: Considering the MICs and disk diffusion results, the ginseng extract (64,128 mg.ml-1) shows considerable antifungal effects compared to Itraconazole.