The paper is focused on appreciation of differentiation of demographic development in CR regions. There are defined key factors of essential influence in a demographic situation on the basis of principal component analysis. With use cluster analysis there were created groups of regions with similar development. Analysis of basic regional differences follows.Pieces of knowledge introduced in this paper resulted from solution of an institutional research intention MSM 6046070906 „Economics of resources of Czech agriculture and their efficient use in frame of multifunctional agri-food systems“.