The number of students expanding their academic programs to include learning experiences abroad has been steadily increasing over the past two decades. This study investigates the cultural identification of short-term study abroad participants and its influence on their acculturation process. By means of a widely used acculturation scale, it was found that there were statistically significant effects of cultural experiences on cultural identification, specifically decreases in cultural identification scores. Implications from this study suggest a deeper understanding of students’ ethnic identifications can be helpful in developing study abroad programs that promote the goals of student awareness, cultural sensitivity, and global competence.