Liangshan Yi Autonomous Prefecture locates at the south of Sichuan, China, and this area has the largest population of Yi ethnic nationally. In order to study the population data of Y-STRs of this area and contribute to the worldwide Y-STR database, we genotyped 628 unrelated individuals using commercially available Goldeneye® DNA ID 27Y system. Gene diversity (GD) values and haplotype diversity (HD) were calculated. Population comparison results showed that Liangshan Yi population was significantly differently from other populations.