Background: This study analysis the epidemiological characteristics of incidence, gender, age, occupation, educational level, income, residence location, and timescale of 773 pulmonary tuberculosis patients in Hulin County in North China from 2014 to 2016. Methods: Two hundred and sixty-seven strains were identified and nine drug sensitivity tests against isoniazid (INH), streptomycin (S), ethambutol (EMB), rifampicin (RFP), ofloxacin (OFX), capreomycin, prothionamide, para-aminosalicylic acid (PAS), and kanamycin. Results: The top five drug resistance sequences of nine kinds of anti-TB drugs were S (24.535%) >INH (13.383%) >RFP (8.178%) >OFX (4.089%) >EMB (1.859%). Conclusion: The anti-TB medicine needs to be use under the guidelines to minimize drug resistance, and the elder age and poor also the work focus in the future.