Some new kinds of antibiotics-free antibacterial agents are required to deal with bacterial infections due to the occurrence of drug-resistance. In this work, Cu-based metal-organic framework (HKUST-1) embedded with CuS NPs were fabricated via a simple in-situ sulfuration process. The synthesized MOFs exhibited an highly effective disinfection efficacy of 99.70 % and 99.80 % against Staphylococcus aureus and Escherichia coli within 20 min irradiation of near-infrared (NIR) light, respectively, which was ascribed to the cooperative effects of photodynamic and photothermal effects of the composites. A certain amount of Cu ions of the MOFs were reacted to form CuS NPs, which endowed this composite with outstanding photocatalytic and photothermal performance during NIR light irradiation. Moreover, HKUST-1 that composed of low toxic organic ligand 1,3,5-benzenetricarboxylic acid (HBTC) coordinating copper ions could be a controllable carrier that imposed certain constraint on the NPs. Hence, these CuS@HKUST-1 would be a promising bioplatform for rapid bacteria-killing.