In this study, we focus on the measurement of different nitrogen (N) forms and investigate the spatial-temporal variability of degradation coefficient in river channels. We aim to provide a new approach of deriving in-situ degradation coefficients of different N forms, and highlight factors that determine the spatial-temporal variability of degradation coefficients. Our results are based on a two-year field survey in 34 channels around the Taihu Lake Basin, eastern China. The derived degradation coefficients of different N forms based our newly-developed experimental device are: degradation coefficients of TN, NH-N and NO-N range from 0.006-0.449 d, 0.022-1.175 d and -0.096-2.402 d, respectively. The degradation coefficients of N show strong dependence on N concentration and water temperature. The seasonal difference of water temperature and N concentration leads to spatial-temporal variability of degradation coefficients. The derived degradation coefficients of N are further verified through one-dimensional water quality model simulations. The degradation coefficient obtained in this study and the influencing factors of its spatial-temporal variability provide invaluable reference for studies in aquatic environment.