This study evaluated the safety of and humoral response to the Merial Recombitek recombinant canine distemper virus (rCDV) vaccine in maned wolves ( = 9, age 2-9 yr). All maned wolves had prior history of annual vaccination with the Merial Purevax ferret rCDV vaccine. Serum neutralization (SN) to CDV was measured prior to initial vaccination with the rCDV Recombitek vaccine followed by a booster vaccination at 4-6 wk. Final SN titers were obtained at 13 wk post initial vaccination. The maned wolves developed no observable adverse side effects through the study. Pre-Recombitek vaccination SN titers ranged from negative to 1: 8. Postvaccination CDV titers ranged from negative to 1: 8, and were therefore below the range of that considered protective in domestic dogs.