In this paper, a high-temperature low-cycle fatigue life prediction model, based on the total strain energy density method, was established. Considering the influence of the Masing and non-Masing behavior of materials on life prediction, a new life prediction model was obtained by modifying the existing prediction model. With an 800H alloy of the heat transfer tube of a steam generator as the research object, the high-temperature and low-cycle fatigue test was carried out at two temperatures. The results show that the predicted and experimental results are in good agreement, proving the validity of the life prediction model.