Molecularly imprinted polymers (MIPs) have been developed to replace antibodies for the recognition of target molecules (such as antigens), and have been integrated into electrochemical sensing approaches by polymerization onto an electrode. Electrochemical sensing is inexpensive and flexible, and has demonstrated utility in point-of-care devices. In this work, several 2D (conductive) materials were employed to improve the performance of MIP sensors. Screen-printed electrodes were coated by the electropolymerization of aniline and metanilic acid, commingled with target molecules and various 2D materials. Tungsten disulfide (WS) with an average particle size of 2 μm was found to increase the sensitivity of detection of molecularly imprinted conductive polymer-coated electrodes to 17β-estradiol. As estradiol concentrations are important to eel aquaculture, we screened eel serum samples to determine their 17β-estradiol concentrations, which were found to be in the range 28.2 ± 3.6 to 73.0 ± 11.6 pg/mL after dilution. These results were in agreement with measurements using commercial immunoanalysis.