A high performance versatile composite hollow fiber nanofiltration (NF) membrane is reported for the separation of glyphosate from saline waste streams. Preparation of SPEEK based on an amorphous poly (ether ether ketone, PEEK) was investigated. The membrane was prepared by coating sulfonated polyether ether ketone (SPEEK) onto a polyethersulfone (PES) ultrafiltration (UF) hollow fiber membrane. The composite membrane was characterized by water permeability, scanning electron microscopy, and rejection toward sodium sulfate (Na₂SO₄), sodium chloride (NaCl), and calcium chloride (CaCl₂). About 90% rejection toward sulfate anions and only 10% rejection for calcium cations were obtained. A water permeability around 10-13 LMHBar and 90% rejection for polyethylene glycol (PEG) with a molecular weight of 4000-6000 Da were observed. In the separation of glyphosate from saline wastewater, the membrane rejected less than 20% of NaCl and higher than 90% of glyphosate at an operating pressure of 5 bars and pH = 11.0. An economic analysis indicated that the cost for recovery of glyphosate was comparably low to the value gained by an increase in the productivity. The results may lead to a new promising low energy solution for the environmental problem faced by the herbicide industry.