The purpose of this study was to assess the effect of canola oil and soybean oil on production performance and serum lipid profile in layers. In this study 15 experimental units (8 layers per experimental unit) were randomly allotted to 5 different dietary treatments viz control (A). containing 2.5 % canola oil (B), 5% canola oil (C), 2.5% soybean oil (D) and 5% soybean oil (E) for a period of 9 weeks. Effects of five treatments on production parameters including egg production, egg quality, weight gain and serum lipid profile, serum cholesterol, triglycerides, low-density lipoprotein and high-density lipoprotein were monitored. Serum lipid profile was determined 0.31 and 63 days from start of experiment. Significantly (P<0.05) less serum cholesterol was found in treatment C (295.1 mg/dl) as compared with treatment A (321 mg/dl). Low density) lipoprotein cholesterol (LDL) was significantly (P<0.01 ), less in treatment C ( 131.7 mg/dl) as compared with treatment A. ( 161 mg/dl) and high density lipoprotein cholesterol (HDL) was significantly (P<0.01 ) high in treatment C (31.76 mg/dl) as compared with treatment A (25.42 mg/dl) and triglyceride (TG) was found significantly (P<0.01 ) less in treatment E ( 907.3 mg/dl) as compared with treatment A (960 mg/dl). The results suggested that as the percentage of oils increased in the diet, serum lipid profile showed a positive trend.