Objective To investigate the role of ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 (ASAP1) in the infection of murine macrophages RAW264.7 with Mycobacterium tuberculosis, and the function of ASAP1 in macrophage migration. Methods The expression of ASAP1 was down-regulated in RAW264.7 cells using siRNA, and then the cells were infected with H37Ra. The intracellular bacterial load was determined by fluorescence confocal microscopy and colony forming unit (CFU), and the migration ability of the cells were evaluated by Transwell assay. Results Compared with the control cells, the intracellular bacterial load was significantly enhanced in ASAP1-knockdown cells, whereas the migration ability of cells was reduced after knockdown of ASAP1 expression. Conclusion ASAP1 is important for RAW264.7 cell migration, and down-regulated ASAP1 expression may impair the migration ability of RAW264.7 cells.