Freshwater mudsnail (Potamopyrgus antipodarum) estrogen receptor: identification and expression analysis under exposure to (xeno-)hormones.
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Freshwater mudsnail (Potamopyrgus antipodarum) estrogen receptor: identification and expression analysis under exposure to (xeno-)hormones.

Stange, Daniela;Sieratowicz, Agnes;Horres, Ralf;Oehlmann, Jörg;
Ecotoxicology and environmental safety 2012 Vol. 75 pp. 94-101
608
stange2012freshwaterecotoxicology

Abstract

Molluscs are raising attention as ecotoxicological test organisms due to their high diversity and ecological importance. The ovoviviparous prosobranch gastropod Potamopyrgus antipodarum (freshwater mudsnail) responds very sensitively to xenobiotics and has therefore been proposed as OECD standard test organism. Endocrine disrupting chemicals influence the reproduction of P. antipodarum, which can be assessed by embryo numbers in the brood pouch. However, the knowledge about the endocrine system of P. antipodarum is rather limited. The aim of this study was to identify an estrogen receptor in the endocrine system of P. antipodarum and to investigate if this receptor is differentially expressed under exposure to (xeno-)hormones (17α-ethinylestradiol, bisphenol A and 17α-methyltestosterone). The DNA-binding domain of the identified ER-like transcript has an amino acid identity of 92 percent compared to the ER of the gastropod Nucella lapillus (84 percent to human ERα) and 83 percent in the ligand binding domain (38 percent to human ERα). Furthermore, the P. antipodarum ER is transcriptionally regulated as shown by quantitative real-time PCRs of (xeno-)hormone exposed snails. 17α-ethinylestradiol and bisphenol A exposure resulted in a transitory ER-mRNA increase while17α-methyltestosterone caused a transitory reduction of ER-mRNA. In addition the solvent dimethyl sulfoxide had also a modulating effect on the receptor.

Keywords

NMR HPLC sodium carboxymethyl cellulose SEM metabolic disease high-performance liquid chromatography gst ampk nox mtor traditional chinese medicine thin layer chromatography glutathione-s-transferase nf-κb mammalian target of rapamycin nuclear magnetic resonance dimethyl sulfoxide fcs pbs fetal calf serum peroxisome proliferator-activated receptor gamma tlc bovine serum albumin dmso bmp natural product bsa anova srebp pparγ dmem phosphate buffered saline lbd 3-isobutyl-1-methylxanthine amp-activated kinase atcc american type culture collection badge cmcna dulbecco's modified eagle's medium ec(50) egfp ibmx mef nadph-dependent oxidase nbs peroxisome proliferator-activated receptor rxr spf tcm tr-fret analysis of variance bisphenol a diglycidyl ether bone morphogenic protein 4 effective concentration 50% enhanced green fluorescent protein ligand-binding domain mouse embryonic fibroblasts newborn bovine serum nuclear factor κb retinoic x receptor specific pathogen free standard error of the mean sterol regulatory element binding protein time-resolved fluorescence resonance energy transfer

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DOI:
10.1016/j.ecoenv.2011.09.003

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74043
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