EDCs raised a certain concern for living health began since last century, via interfere with natural hormone functions and produce reversible or irreversible biological effects. Bisphenol (BPA) is an organic compound that causing human health risks. Different bacterial spp. has biodegradation ability for wide range of EDC. 4 bacterial spp. were isolated and worked on (Pseudomonas auroginosa, Pseudomona lutoi, Proteus penneri, and E.coli). The effect of substrate tolerate bisphenol (5 mg/ ml) using the well diffusion method were investigated. The biodegradation effect of bacterial isolated on breakdown bisphenol and its derivatives using UV vis spectrophotometer were studied and comparing in various incubation time and temperature to assess the effect of the physical conditions on the bacterial ability of bisphenol degradation. Proteus penneri showed a significant ability to resistance to para-phenol and meta-phenol, while highly sensitive to ortho- aminophenol and para- creso, Pseudomonas aeroginosa and Pseudomonas lutiola sensitive to meta- aminophenol, while resist to degraded three phenol compounds (para, ortho aminophenol, and para crysol). Data showed a significant effect for ps. Lutiola after 15 days of incubation followed by p. penorri and E.coli. to degraded phenol chemical compounds. Data demonstaed that bacterial spp. ps. Lutiola has an obvious degradation effect for bisphenol compound after 15 days of incubation however, Sp. euroginosa showed an absolutely different behavior toward bisphenol which showed an raising absorbance after the 15 days of incubation. The ability of different local bacterial isolated to breakdown the phenol and its derivitives in surface water was investigated .