This review (with 116 refs.) summarizes the state of the art in resonance Rayleigh scattering (RRS)-based analytical methods. Following an introduction into the fundamentals of RRS and on the preparation of metal nanoparticles, a first large section covers RRS detection methods based on the use of gold nanoparticles, with subsections on proteins (albumin, bovine serum albumin and ovalbumin, glycoproteins, folate receptors, iron binding-proteins, G-proteins-coupled receptors, transmembrane proteins, epidermal growth factor receptors), on pesticides, saccharides, vitamins, heavy metal ions (such as mercury, silver, chromium), and on cationic dyes. This is followed by a section on RRS methods based on the use of silver nanoparticles, with subsections on the detection of nucleic acids and insecticides. Several Tables are presented where an RRS method is compared to the performance of other methods. A concluding section summarizes the current status, addresses current challenges, and gives an outlook on potential future trends. Graphical Abstract Change in the resonance Rayleigh scattering (RRS) intensity when mixing the nanoparticles with the specific analyte.