The L-ascorbate peroxidase 6 gene () is one of the most important genes for scavenging HO and plays a vital role in plant resistance to environmental stresses. In this study, a novel gene (GenBank Accession No. KT907352) was obtained from a sugarcane variety (ROC22). Bioinformatics analysis showed that ScAPX6 has a cDNA length of 1,086 bp and encoded 333 amino acid residues. Subcellular localization confirmed that ScAPX6 was located in the chloroplast. Enhanced growth of BL21 cells that expressed ScAPX6 showed high tolerance under copper (Cu) stress. Real-time quantitative PCR analysis revealed that was constitutively expressed wherein with the highest expression levels in sugarcane pith and leaf and the lowest in the root. was down-regulated by salicylic acid (SA), hydrogen peroxide (HO), polyethylene glycol (PEG) and sodium chloride (NaCl) stimuli. Interestingly, it was significantly up-regulated under the stresses of abscisic acid (ABA) and methyl jasmonate (MeJA) wherein with the highest inducible expression levels at 6 h at 6.0- and 70.0-times higher, respectively than that of control. Overexpression of in leaves enhanced the resistance to the infection of tobacco pathogens and var. . These results implied that might positively respond to ABA, MeJA, and Cu, but might negatively respond to the stresses of SA, HO, PEG, and NaCl. Keeping in view the current investigation, could be associated with the hypersensitive response (HR) or immunity of sugarcane, which will provide a baseline for the function identification of sugarcane .