Peripheral fatigue results from multiple electrochemical and mechanical events in the cell body and the muscle-tendon complex. Combined force and surface electromyographic signal analysis is among the most widely used approaches to describe the behaviour of a fatigued muscle. Advances in technologies and methodological procedures (e.g. laser diffraction, P magnetic resonance spectroscopy, shear-wave elastography, tensiomyography, myotonometry, mechanomyography, and high-density surface electromyography) have expanded our knowledge of muscle behaviour before, during, and after a fatiguing task. This review gives an update on recent developments in technologies for investigating the effects of peripheral fatigue linked to skeletal muscle contraction and on mechanistic insights into the electrochemical and mechanical aspects of fatigue. The salient points from the literature analysis are: (1) the electrochemical and mechanical events in the cell (alterations in cross-bridge formation and function and in depolarization of the tubular membrane) precede the events taking place at the muscle-tendon complex (decrease in muscle-tendon unit stiffness); (2) the changes in the fatigued muscle are not homogenous along its length and width but rather reflect a functional compartmentalisation that counteracts the decline in performance; (3) fatigue induces changes in load sharing among adjacent/synergistic muscles. A focus of future studies is to observe how these regional differences occur within single muscle fibres. To do this, a combination of different approaches may yield new insights into the mechanisms underlying muscle fatigue and how the muscle counteracts fatigue.