The present study was designed to investigate the antagonistic activity of BL350-2 against mycotoxigenic strains of and . In vitro coincubation for 5 days indicated BA1 as the most sensitive strain, with a growth inhibition of 62%, followed by MG7 (60%), MC12 (53%), MC05 (50%), CM5 (49%), SB01 (47%), and MD1 (44%). Likewise, the majority of the tested strains on exposure to bacterial volatiles showed complete inhibition of mycotoxin synthesis. In vivo assays on maize ears resulted in 88% reduction in CM5 growth and complete inhibition of fungal sporulation and aflatoxin accumulation. The GC-MS-based volatile profile showed 3-methyl-1-butanol as the most abundant compound. The findings of the present study advocate that BL350-2 is suitable as a biocontrol agent against mycotoxigenic fungi, at least during storage of cereal grains.