Studies show that mental disorders are highly prevalent among students. Therefore, the present study aimed to examine the role of mindfulness and spiritual intelligence in the students’ mental health studying at university of medical sciences. The study population included all undergraduate and medicine students. A total of 393 female and male students (193 medical and 200 non-medical students) were selected through randomly. General Health Questionnaire (GHQ) and spiritual intelligence and mindfulness questionnaire were used to evaluate the participants. The results revealed the negative correlation of mental health with mindfulness and spiritual intelligence and a positive correlation between mindfulness and dimensions of spiritual intelligence. Also, the dimension of spiritual life (43.1%) and mindfulness (31%) had a significant negative effect on the explained variance of the students’ mental health. Analysis of variance showed that the scales of mindfulness, perception of existence, somatic symptoms, and anxiety were higher among women. Therefore, the students can be more capable of coping with existing traumas and pressures by boosting their spirituality, consciousness, and mindfulness.