Appropriate combinations of LAB strains should be selected to optimize the ensiling process, and the additives should be adjusted to the ensiled forage crops. The aim of this study was to determine the effect of inoculation with three Lactobacillus species on the chemical parameters and microbiological quality (beneficial and harmful microbiota) of grass silage.Three species: L. paracasei-LPa, L. brevis-LB and L. plantarum-LPl, isolated from sugar beet silage and characterized based on 16S rDNA sequences and biochemical parameters, were analyzed in the study. Single strains and their combinations were used as silage inoculants. The basic chemical and microbiological (qPCR) parameters of silages were determined. Based on the results of AHC and PCA, it was determined that silages inoculated with single LAB strains and LPa+LB and commercial additive (0+) were characterized by the best quality, particularly with regard to microbiological parameters and as the only one from the tested variants, it lowered the pH significantly. A consortium of three Lactobacillus species had no influence on silage quality, whereas LPa+LB and LPl+LB combinations as well as a commercial additive exerted positive effects. Inoculation inhibited the growth of toxin-producing fungi.Only the appropriate LAB composition can improve the quality of the ensiled material (antagonistic relationship). Only combination LPa+LB was able to improve the value of low dry silage, nevertheless, almost all combinations were able to reduced concentrations of toxin-producing fungi. This article is protected by copyright. All rights reserved.