In this study, the treatment of kitchen wastewater was demonstrated using microbial fuel cell (MFC) consisting photosynthetic microorganism as cathode catalyst. The power density and biomass generation were investigated using two photosynthetic microorganisms namely Synechococcus sp. and Chlorococcum sp., respectively. Cyclic Voltammogram analysis was performed to study the exoelectrogenic activity of mixed culture microorganisms present in the wastewater. The MFC experimental results showed that both species influenced the power production and COD removal efficiency. The MFC observed the higher power density of 41.5 ± 1.2 mW/m with Synechococcus sp. as compared to Chlorococcum sp. (30.2 ± 0.8 mW/m). The effect of CO supply, light intensity and wastewater COD concentration on MFC performance were investigated. This study demonstrated the possibility of bioelectricity generation, CO sequestration and biomass production with the algae biocatholyte during the treatment of kitchen wastewater in the MFC.