Carbapenem-resistant Klebsiella pneumoniae isolates (carrying the carbapenemase gene bla) of sequence type 16 caused hospital-acquired bloodstream infection or gut colonization in two ICU patients. We hypothesized that handwashing sinks were the source and all handwashing sinks in the ICU were sampled. Whole genome sequencing and analysis revealed that one sink was the source of CRKP colonization/infection in the two patients instead of direct transmission of a common clone between patients. This study highlights sinks as an important source of multidrug-resistant organisms. Sink management including prohibition of disposal body fluid and daily disinfection with chlorine curbed the transmission.