In this study, a novel ecofriendly chitosan- silver nanoparticles hybrid was developed. Biological method using leaf extract of T. portulacifolium was used as reducing agent for its synthesis and the antibacterial efficiency of these hybrid nanoparticles were evaluated against the bacteria E. coli and S. marcescens organisms. The intense peak observed around 419 nm in the UV-Vis indicates the formation of silver nanoparticles. The XRD analysis showed that the hybrid chitosan-silver nanoparticles have a polycrystalline and face-centered cubic configuration. FTIR spectrum hybrid chitosan-silver nanoparticles indicated speaks vibration of NH and OH. The EDS analysis confirmed the presence of Ag, O, C and N elements in the prepared sample. The spherical shape was obtained from TEM analysis and it indicated that with average particles around 3.24 nm to 44.80 nm. The prepared hybrid chitosan-silver nanoparticles showed significant antibacterial activities against E. coli and S. marcescens. In addition, the surface membrane damages and surface morphology of test pathogens were visualized using FESEM analysis.