The aim of this study is to identify the effects of servant leadership qualities of managers on organizational citizenship behavior in hotel enterprises. In the scope of the research, the data was obtained from 513 employees in Istanbul and Afyonkarahisar in 2013 by employing questionnaire techniques. As a result of data analysis, a moderate positive relationship between servant leadershipand organizational citizenshipbehavior was detected. In this context, it is found that while empowerment, vision and trust behaviors influence employees’ organizational citizenship behavior positively, no positive impact of agapao love and altruism behavior on organizational citizenship behaviorwas detected. It is seen that, the perceptions of employees on the trust which is one of sub-dimensions of servant leadership and the level of organizational citizenship behavior perceptions in Istanbul is more positive than employees in Afyonkarahisar. In addition, while a significant difference is identified in employees’ servant leadership perceptions according to their education levels, no significant difference was observed in consideration with their other individual characteristics.