A novel plasmid carrying carbapenem-resistant gene blaKPC-2 in Pseudomonas aeruginosa Yan-Yan Hu, Qi Wang, Qiao-Ling Sun, Gong-Xiang Chen, Rong Zhang2nd Affiliated Hospital of Zhejiang University, School of Medicine, Zhejiang University, Hangzhou, People’s Republic of ChinaAbstract: A carbapenem-resistant Pseudomonas aeruginosa strain PA1011 (ST463) was isolated from a patient in a surgical intensive care unit. PCR detection showed that PA1011 carried the blaKPC-2 gene. A plasmid was isolated and sequenced using the Illumina NextSeq 500 and PacBio RSII sequencing platforms. The plasmid was named pPA1011 and carried the carbapenem-resistant gene blaKPC-2. pPA1011 was a 62,793 bp in length with an average G+C content of 58.8%. It was identified as a novel plasmid and encoded a novel genetic environment of blaKPC-2 gene (ΔIS6-Tn3-ISKpn8-blaKPC-2-ISKpn6-IS26).Keywords: plasmid, genetic environment, KPC, ST463, Pseudomonas aeruginosa