This study was conducted to investigate the relationship of workplace incivility experienc and organizational justice with instigated workplace incivility with regard to the mediation role of negative affect and moderator role of neuroticism. The statistical population was 411 employees from a service organization. Out of 411, 198 employees were selected by simple random sampling as Sampeles. The instruments consisted of Experienced Workplace Incivility Scale (Cortina, etal, 2001), Organizational Justice Questionnaire (Colquitt, 2001), Job-related Affective Well-being Scale (JAWS) (Van Katwyk, etal, 2000), Instigated Workplace Incivility Scale (Blau & Andersson, 2005) and NEO Personality Inventory (McCrae & Costa, 1985). For data analysis, mediation analysis in Preacher and Hayes (2008) SPSS Macro program and moderation analysis in Hayes and Mattes (2009) program was used. Result indicated that experienced workplace incivility, interpersonal justice, informational justice, neuroticism and negative affect were correlated with instigated workplace incivility (P<0.01). Furthermore negative affect mediated the relation between interactional justice and instigated incivility (P<0.05). The results showed victims of incivility retaliate this behavior with instigated incivility and neuroticism moderated this relationship. In the other hands, the relationship between experienced incivility and instigated incivility were stronger for individuals high in neuroticism than individuals low in neuroticism (P<0.01).