Cell walls are basically complex with dynamic structures that are being involved in several growth and developmental processes, as well as responses to environmental stresses and the defense mechanism. Pectin is secreted into the cell wall in a highly methylesterified form. It is able to perform function after the de-methylesterification by pectin methylesterase (PME). Whereas, the pectin methylesterase inhibitor (PMEI) plays a key role in plant cell wall modification through inhibiting the PME activity. It provides pectin with different levels of degree of methylesterification to affect the cell wall structures and properties. The PME activity was analyzed in six tissues of , and found a high level in the leaf and leaf sheath. PMEI families have been identified in many plant species. Here, a total of 55 pectin methylesterase inhibitor genes () were identified from whole genome, a more detailed annotation of this crop plant as compared to the previous study. Chromosomal localization, gene structures and sequence characterization of the family were analyzed. Moreover, -acting elements analysis revealed that each gene was regulated by both internal and environmental factors. The expression patterns of each gene were also clustered according to expression pattern analyzed in 47 tissues under different developmental stages. Furthermore, some were induced when treated with hormonal and abiotic stress. Taken together, these results laid a strong foundation for further study of the functions of and pectin modification during plant growth and stress responses of cereal.