Nursing shortage is a major global concern about healthcare. In this regard, nurses' organizational commitment is an important issue that should be focused on. Since limited data are available about the factors associated with nurse organizational commitment in a nursing healthcare context, further research is needed. This study aimed to examine the influence of job satisfaction and work environment on the organizational commitment of nurses. A predictive design was employed. The study sample consisted of nurses at a university hospital in Turkey (n = 595). The study data were analyzed using descriptive statistics, Pearson correlation, and multiple regression analyses. A positive significant relationship was found between job satisfaction, work environment, and organizational commitment. The variables altogether explained 63% of nurses' organizational commitment and significant predictors were "self-realization" (p = 0.000), "nurses' participation in management and representative power" (p = 0.000), "nurse managers' attitudes and leadership qualities" (p = 0.007), "general quality" (p = 0.01), and "communication among physicians, nurses and colleagues" (p = 0.03). The development of strategies for increasing job satisfaction among nurses and creating positive work environments may lead administrators to increase organizational commitment in helping to the problem of nursing shortage. Nursing work environment and job satisfaction have been identified as the predictors of nurses' organizational commitment in the Turkish healthcare context.