In order to study the competition and spatial association of trees in mixed Hornbeam stands, two 1-ha sample plots (100m×100m) were selected in the reserve area of Asalem forests in Guilan. In each plot, species and diameter at breast height (DBH) of all trees with a diameter > 7.5 cm were recorded and mapped using slope-corrected distance and azimuth from southwest corner of each plot. Distances and azimuths were later transformed to Cartesian coordinates, and all of the measured trees were assigned to one of four diameter size classes: ST (Small Timber, (DBH ≤ 32.5 cm), MT (Medium Timber, 32.5 < DBH ≤ 52.5 cm), LT (Large Timber, 52.5 < DBH ≤ 72.5 cm) and ELT (Extra-Large Timber, DBH > 72.5). Spatial patterns of the entire trees as well as the spatial associations among tree size classes (Intra-specific) and among species (Inter-specific) were analyzed by univariate and bivariate Ripley’s K-function. Results showed that the two stands exhibited a clustered distribution. Furthermore, the interaction between the ST class and other classes was significant (P0.05). The inter-specific investigation revealed that Fagus orientalis and Carpinus betulus trees tend towards an independent association at 1-20 meter scales. At higher scales they showed yet a negative correlation. Moreover, F. orientalis was significantly and negatively correlated to Alnus subcordata at small scales. However, their mutual intra-specific and inter-specific effects were different due to the fact that different natural stands have variety of demands during their life cycle. Therefore, it may not necessarily conform to their response to natural processes. This study concludes a founded understanding of spatial pattern of stands to be a helpful indicator for managing forests based on the currently operational close- to- nature silvicultural system.